Microgénomique/Cellule unique

Tri cellulaire, microdissection et capture laser, single–cell



Thibaud Parpaite, Lucie Brosse, Nina Séjourné, Amandine Laur, Yasmine Mechioukhi, Patrick Delmas, Bertrand Coste, Patch-seq of mouse DRG neurons reveals candidate genes for specific mechanosensory functions, Cell Reports, Volume 37, Issue 5,2021, 109914, ISSN 2211-1247, (Patch-clamp single cell, Smart-seq, Illumina)


Hirbec H.; Marmai C. ; Richard I.; Roudert C. ; Esclangon A. ; Croze S. ; Lachuer J. ; Peyroutou R. ; Rassendren François.  The microglial reaction signature revealed by RNAseq from individual mice » Glia. 2018 May;66(5):971-986. (FACS cell sorting and RNA-seq).

Kratzer I, Strazielle N, Saudrais E, Mönkkönen K, Malleval C, Blondel S, Ghersi-Egea JF. Glutathione Conjugation at the Blood-CSF Barrier Efficiently Prevents Exposure of the Developing Brain Fluid Environment to Blood-Borne Reactive Electrophilic Substances. J Neurosci. 2018 Apr 4;38(14):3466-3479 PMID: 29507144 (laser capture microdissection, transcriptome, RT-PCR).

Guillemin A, Richard A, Gonin-Giraud SGandrillon O. Automated cell cycle and cell size measurements for single-cell gene expression studies. BMC Res Notes. 2018 Feb 1;11(1):92 (C1 single cell autoprep system and PCR).

Donega V, Marcy G, Lo Giudice Q, Zweifel S, Angonin D, Fiorelli R, Abrous DN, Rival-Gervier S, Koehl M, Jabaudon D, Raineteau O. Transcriptional Dysregulation in Postnatal Glutamatergic Progenitors Contributes to Closure of the Cortical Neurogenic PeriodCell Rep. 2018 Mar 6;22(10):2567-2574.(C1 single cell autoprep system and RNA-seq).

Charoy C, Dinvaut S, Chaix Y, Morlé L, Sanyas I, Bozon M, Kindbeiter K, Durand B, Skidmore JM, De Groef L, Seki M, Moons L, Ruhrberg C, Martin JF, Martin DM, Falk J, Castellani V. Genetic specification of left-right asymmetry in the diaphragm muscles and their motor innervation. Elife. 2017 Jun 22;6 (Laser capture microdissection, transcriptome, microarrays) pii: e18481. doi: 10.7554/eLife.18481

Bachy E, Urb M, Chandra S, Robinot R, Bricard G, de Bernard S, Traverse-Glehen A, Gazzo S, Blond O, Khurana A, Baseggio L, Heavican T, Ffrench M, Crispatzu G, Mondière P, Schrader A, Taillardet M, Thaunat O, Martin N, Dalle S, Le Garff-Tavernier M, Salles G, Lachuer J, Hermine O, Asnafi V, Roussel M, Lamy T, Herling M, Iqbal J, Buffat L, Marche PN, Gaulard P, Kronenberg M, Defrance T, Genestier L.
CD1d-restricted peripheral T cell lymphoma in mice and humans.
J Exp Med. 2016 Apr 11 (FACS, transcriptome, microarrays). pii: jem.20150794. [Epub ahead of print] PMID: 27069116.

Foy JP, Tortereau A, Caulin C, Le Texier V, Lavergne E, Thomas E, Chabaud S, Perol D, Lachuer J, Lang W, Hong WK, Goudot P, Lippman SM, Bertolus C, Saintigny P.
The dynamics of gene expression changes in a mouse model of oral tumorigenesis may help refine prevention and treatment strategies in patients with oral cancer.
Oncotarget. 2016 Mar 24. doi: 10.18632/oncotarget.8321.PMID: 27027432 (Laser capture microdissection, transcriptome, microarrays).

Khaznadar Z, Boissel N, Agaugué S, Henry G, Cheok M, Vignon M, Geromin D, Cayuela JM, Castaigne S, Pautas C, Raffoux E, Lachuer J, Sigaux F, Preudhomme C, Dombret H, Dulphy N, Toubert A.Defective NK Cells in Acute Myeloid Leukemia Patients at Diagnosis Are Associated with Blast Transcriptional Signatures of Immune Evasion.
J. Immunol. 2015 Sep 15;195(6):2580-90. (FACS, transcriptome, microarrays) doi: 10.4049/jimmunol.1500262. Epub 2015 Aug 5

Havis E, Bonnin MA, Olivera-Martinez I, Nazaret N, Ruggiu M, Weibel J, Durand C, Guerquin MJ, Bonod-Bidaud C, Ruggiero F, Schweitzer R, Duprez D
Transcriptomic analysis of mouse limb tendon cells during development
Development. 2014 Oct;141(19):3683-96. (FACS, trancriptome, microarrays

Robert VJ, Mercier MG, Bedet C, Janczarski S, Merlet J, Garvis S, Ciosk R, Palladino F.
The SET-2/SET1 Histone H3K4 Methyltransferase Maintains Pluripotency in the Caenorhabditis elegans Germline.
Cell Rep 2014 Oct 23;9(2):443-50. (Macrodissection, transcriptome, microarray

Ingallinesi M, Le Bouil L, Faucon Biguet N, Do Thi A, Mannoury la Cour C, Millan MJ, Ravassard P, Mallet J, Meloni R .
Local inactivation of Gpr88 in the nucleus accumbens attenuates behavioral deficits elicited by the neonatal administration of phencyclidine in rats.
Mol Psychiatry. 2014 Aug 26.] (Laser capture microdissection, transcriptome, RT-PCR

Dkhissi-Benyahya O, Coutanson C, Knoblauch K, Lahouaoui H, Leviel V, Rey C, Bennis M, Cooper HM
The absence of melanopsin alters retinal clock function and dopamine regulation by light
Cell Mol Life Sci. 2013 Apr 19. (Laser capture microdissection, transcriptome, QRTPCR

Desestret V, Riou A, Chauveau F, Cho TH, Devillard E, Marinescu M, Ferrera R, Rey C, Chanal M, Angoulvant D, Honnorat J, Nighoghossian N, Berthezène Y, Nataf S, Wiart M.
In vitro and in vivo models of cerebral ischemia show discrepancy in therapeutic effects of m2 macrophages
PLoS One. 2013 Jun 25;8(6):e67063. (Petite quantité d’ARN, transcriptome, PCR

Genevois AL, Ichim G, Coissieux MM, Lambert MP, Lavial F, Goldschneider D, Jarrosson-Wuilleme L, Lepinasse F, Gouysse G, Herceg Z, Scoazec JY, Tauszig-Delamasure S, Mehlen P.
Dependance receptor TrkC is a putative colon cancer tumor suppressor
Proc Natl Acad Sci U S A. 2013 Feb 19;110(8):3017-22 (Laser capture microdissection, transcriptome, qRTPCR

Ventre E, Brinza L, Schicklin S, Mafille J, Coupet CA, Marçais A, Djebali S, Jubin V, Walzer T, Marvel J.
Negative regulation of NKG2D expression by IL-4 in memory CD8 T cells
J Immunol. 2012 Oct 1;189(7):3480-9. Epub 2012 Aug 31. (FACS, transcriptome, microarray

Tatiana Dupinay, Agnès Nguyen, Séverine Croze, Fabienne, Barbet, Catherine Rey, Patrick Mavingui, Michel Pépin, Joël Lachuer and Catherine Legras-Lachuer
Next-generation sequencing of ultra-low copy samples: From clinical FFPE samples to single-cell sequencing
Current Topics in Virology, 2012 Vol.10, 63 – 83. (NGS)

Varrin-Doyer M, Nicolle A, Marignier R, Cavagna S, Benetollo C, Wattel E, Giraudon P.
Human T lymphotropic virus type 1 increases T lymphocyte migration by recruiting the cytoskeleton organizer CRMP2
J. Immunol 2012 Feb 1;188(3):1222-33. Epub 2012 Jan 6. (FACS, transcriptome, microarray

Rey C., Wierinckx A., Croze S., Legras-Lachuer C. and Lachuer J.
Unraveling more of single cell genomics : micro and nanotools. Royal society of chemistry: nanoscience and nanotechnology
2010 (Eds Bontoux, Dauphinot, Potier) (Laser capture microdissection)

Perrin V., Drouet V., Hassing R., Dufour N., Auregan G., Alves S., Brouillet E., Luthi-carter R., Hantraye P., Déglon N.
Effects of long term and global huntingtin silencing
Annals of neurology, 2009,Mar;65(3):276-85

Kress E, Rezza A, Nadjar J, Samarut J, Plateroti M.
The frizzled-related sFRP2 gene is a target of thyroid hormone receptor alpha1 and activates beta-catenin signaling in mouse intestine
J Biol Chem. 2009 Jan 9;284(2):1234-41 (transcriptome, Laser capture microdissection, microarray

Bernet A., Mazelin L, Coissieux MM., Gadot N., Ackerman SL., Scoazec JY., Mehlen P.
Inactivation of the UNC5C netrin-1 receptor is associated with tumor progression in colorectal malignancies
Gastroenterology, 2007,133 1840-1848. (transcriptome, Laser capture microdissection, qRTPCR

Ducray F. A. Wierinckx, C. Rey, C. Legras, MF Belin, J. Honnorat, and J. Lachuer
Microgenomic: Use of CodeLink human genome bioarrays for gene expression profiling of microdissected cells.
Discovery Matters, (General Electric Healthcare) 2006 (Issue3). (transcriptome, Laser capture microdissection, microarray)